Liu's Haematocyte Stain

 

Intended use:

This kit is for classification of hematocytes.

 

Principle:

Liu’s Stain is modified from the Romanowsky Stain technique. Staining of cells involves physical adsorption and chemical affinity which allows stain to penetrate and remain within cells. Because each cell and its components are different in chemical composition, their affinity to acid stain (Eosin) and alkaline stain (Methylene blue) of this kit varies significantly. After staining the smear with the Liu’s Stain, different types of cells will appear in different colors. Consequently, one can identify cells based on their stain color, shape and other physical characteristics.

 

Methods:

Standard stain

1. Add Liu A Solution (about 0.5~0.8 ml) to stain for 30 seconds.

2. Drop Liu B Solution (twice of Liu A Solution) onto A Solution. Blow solutions with mouth or rubber pipette bulb

    to mix thoroughly . Allow to stain for 90~120 seconds.

3. Rinse with water gently, dry and examine the finished slide under a microscope.

 

Rapid stain

1. After adding Liu A Solution (about 0.5~0.8 ml), immediately drop Liu B Solution (twice of Liu A Solution) onto

    A Solution. Blow solutions with mouth or rubber pipette bulb to mix thoroughly. Allow to stain for 30~60

    seconds.

2. Rinse with water gently, dry and examine the finished slide under a microscope.

 

Specifications:

Contents	6vials x20ml/kit	4Btlsx250ml/kit	4Btlsx250ml/kit	4Btlsx250ml/kit	Components
Liu A Solution	2x20ml	2x250ml	4x250ml	/	Eosin, Methanol
Liu B Solution	4x20ml	2x250ml	/	4x250ml	Methylene blue

 

Precaution:

1. Cap the reagent bottle immediately after use to avoid vaporization.

2. Do not use the reagents beyond the stated expiration date. Store the kit in a cool dry place and avoid direct

    sunlight.