Bacterial Spore Stain

 

Principle:

Bacterial spores are the resting state of bacteria. They can resist various physical and chemical factors and endure execrable conditions. Spores, which have strong refraction, can be observed under light microscope when properly stained. Baso Spore Stain is a very useful tool for bacteria differentiation that allows the identification of size, shape and location of bacterial spores.

 

Specifications:

Contents	3vials×20ml/Kit	Components
Carbolfuchsin	1× 20ml	Carbolfuchsin
Decolorizer	1× 20ml	Ethanol
Alkaline methylene blue	1× 20ml	Methylene blue

 

Methods:

1. Prepare smear of bacteria with spores, air dry and fix on flame.

2. Add Carbolfuchsin on slides. Heat on small flame and keep dye steaming for about 5 minutes. After cooling,

    wash with water.

3. Decolorize with 95% ethanol for 2 minutes and wash with water.

4. Counterstain with Alkaline methylene blue for 30 seconds, followed by water wash. Air dry slides and then

    observe under oil immersion lens.

 

Precaution:

The age of bacteria for spore staining should be controlled and it is best to use bacteria with spores remained attached.